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. 2021 Mar 15;9:618448. doi: 10.3389/fbioe.2021.618448

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Copyright © 2021 Gurrala, Byrne, Brown, Tiongco, Matossian, Savoie, Collins-Burow, Burow, Martin and Lau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Normalizing ECM fiber orientation distributions allows comparison of distinct samples and combination of replicates. (A) Fiber orientation distribution output by DiameterJ for a SEM micrograph of decellularized ECM (Image A) and the same image rotated 90° for which the mean fiber orientation (red line) and orientation set as 0° by DiameterJ (green line) are superimposed. Red stripes indicate the distribution’s mean. (B) Superimposed normalized fiber orientation distributions for Image A and a separate SEM micrograph, Image B, generated by subtracting the mean fiber orientation from all angle orientations, shifting the distributions’ means to 0°.