FIGURE 3.

WEKA-DiameterJ method produces more accurate fiber segmentation than CT-FIRE. (A) Schematic of workflow for quantifying collagen alignment for a scanning electron micrograph (SEM) using the CT-FIRE method and WEKA-DiameterJ method (WEKA+DJ). Segmented fibers are indicated by colored lines on the CT-FIRE Map, by white pixels on the Weka Segmentation Tool (WTS) Map, and as thin black lines on the Axial Thinning (AT) Map. (B) A region of interest within the SEM micrograph and corresponding CT-FIRE Map, and AT Map after segmentation with the WEKA-DJ method. A single foreground fiber (red arrow) within the SEM is oversegmented by CT-FIRE but appropriately segmented by the WEKA-DJ method. Segmentation by CT-FIRE incorrectly segmented fibers within a pore (red box). (C) Incidence of oversegmentation (mean + SEM) by CT-FIRE and WEKA-DJ methods (n = 5) (p = 0.0172).