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Normalized orientation distributions identify visible differences in ECM fiber alignment. (A) SEM micrographs of decellularized ECM generated using breast tissue alone (CTRL) and seeded with MDA-MB-231 cancer cell lines (231). (B) Normalized fiber orientation distributions for CTRL and 231 ECM (n = 14) (p = 0.000114). (C) Orientation index (mean + SEM) for CTRL and 231 ECM (n = 14) (p = 0.6520).
