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. 2021 Mar 11;9:647031. doi: 10.3389/fbioe.2021.647031

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Copyright © 2021 Teixeira, Chaves, Torres, Barrias and Bidarra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fibroblasts and OEC cultured on RGD-alginate porous scaffolds after 14 days observed under CLSM using two different seeding approaches: (A) only on top, and (B) on both sides of the scaffold. Fibroblasts mono-cultures were stained for F-actin (magenta), fibronectin (FN, cyan), and endothelial cells monocultures for laminin (green) and CD31 (red); scale bar = 200 μm. Irrespectively of the approach (C) cells were able to attach to the scaffold and align along the pore walls (black arrows); scale bar = 50 μm. Fibroblasts and OEC were able to produce and assemble endogenous ECM proteins, namely (D) fibronectin (FN) and (E) laminin; scale bar = 50 μm.