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. 2020 Jan 9;223(6):1103–1112. doi: 10.1093/infdis/jiz646

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Immunoblot of porcine protease-resistant prion protein (PrP^res). A, Brain PrP^res Western blot profile in porcine PrP (PoPrP)–Tg001 mice inoculated with the different classic bovine spongiform encephalopathy (BSE)–derived inocula: Ca-BSE 2 (lane 1), Ca-BSE 2/Tg110 Bo6OR (lane 2), Ca-BSE 2/Tg008 Bo5OR (lane 3), BSE in sheep ARQ/ARQ (lane 4), BSE in sheep ARR/ARR (lane 5), BSE in TgOV ARQ (lane 6), BSE in TgOV ARR (lane 7), experimental BSE in goat (lane 8), BSE in goat CH636 (lane 9), BSE in Tga20 (lane 10), BSE in wild-type mouse (lane 11), variant Creutzfeldt-Jakob disease (vCJD) 129 M/M NHBY0/0014 (lane 12), vCJD 129 M/M BC1458 (lane 13), and BSE in HuPrP-Tg340 (lane 14). B, Brain PrP^res Western blot profile in the different inocula and in the PoPrP-Tg001 brain homogenates after in vivo or in vitro experiments. Original inocula used included Ca-BSE/Pig (lane 1), Ca-BSE 2 (lane 4), BSE in Sheep ARQ/ARQ (lane 7), and vCJD 129 M/M BC1458 (lane 10). PoPrP-Tg001 mouse infected with: Ca-BSE/Pig (lane 2), Ca-BSE 2 (lane 5), BSE in Sheep ARQ/ARQ (lane 8) and vCJD 129 M/M BC1458 (lane 11). Brain homogenates from PoPrP-Tg001 were used in protein misfolding cyclic amplification seeded with Ca-BSE/Pig (lane 3), Ca-BSE 2 (lane 6), BSE in sheep ARQ/ARQ (lane 9), and vCJD 129 M/M BC1458 (lane 12). PrP^res was detected using Sha31 monoclonal antibody. (Molecular weight in kilodaltons is shown at the right of each panel.)