Skip to main content
. 2021 Mar 11;9:655889. doi: 10.3389/fcell.2021.655889

FIGURE 1.

FIGURE 1

MYC increases intracellular cholesterol levels and promotes cell proliferation. (A,B) U2OS and HepG2 cells were transfected with control or two different sets of MYC siRNAs. Cells were cultured in the lipoprotein-depleted fetal bovine serum (LPDS) medium. Intracellular cholesterol levels (top) and corresponding protein expression (bottom) were shown. (C,D) U2OS cells and HepG2 cells cultured in LPDS medium were transfected with plasmid expressing the MYC protein. Intracellular cholesterol levels (top) and corresponding protein expression (bottom) were shown. (E,F) Relative cell viability (left) and cell proliferation (right) in control and MYC knockdown U2OS cells and HepG2 cells. Relative cell viability was measured with CCK8. (G,H) Relative cell viability (left) and cell proliferation (right) in control and MYC overexpressing U2OS cells and HepG2 cells. Relative cell viability was measured with CCK8. (I,J) Relative cell viability (left) and cell proliferation (right) of U2OS and HepG2 MYC-knockdown cells cultured in LPDS medium with or without 20 μg/ml cholesterol. Relative cell viability was measured with CCK8. In (A–H), n = 3 independent experiments. Data are means ± SD. Statistical significance was determined by two-tailed unpaired t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.