FIGURE 4.

MYC activates SQLE expression to support tumor growth. (A,B) Relative cell viability (left) and cell proliferation (right) in control and SQLE knockdown U2OS cells and HepG2 cells. Relative cell viability was measured with CCK8. (C,D) Relative cell viability (left) and cell proliferation (right) in control and SQLE overexpressing U2OS cells and HepG2 cells. Relative cell viability was measured with CCK8. (E,F) U2OS and HepG2 cells were transfected with control or MYC siRNA in the presence or absence of exogenous SQLE. Cell viability (left) and cell proliferation (right) were detected, respectively. Relative cell viability was measured with CCK8. (G,H) U2OS and HepG2 cells were transfected with control or SQLE siRNA in the presence or absence of exogenous MYC. Cell viability (left) and cell proliferation (right) were detected, respectively. Relative cell viability was measured with CCK8. In (A–H), 2 μg plasmids and 1 μg siRNAs were used in all experiments. n = 3 independent experiments. Data are means ± SD. Statistical significance was determined by two-tailed unpaired t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.