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. 2021 Jan 4;13:31. doi: 10.1007/s40820-020-00555-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Mitochondrial morphology, membrane potential and fatty acid metabolism in T cells. T cells were treated with aCD3/F/ANs in low-glucose medium supplemented with palmitate as a lipid source. a TEM showing mitochondria of T cells in high-glucose medium, low-glucose medium untreated or treated with aCD3/F/AN. Scale bar: 1 μm (upper panels) and 500 nm (lower panels). b Mitochondrial membrane potential, assessed using MitoTracker Orange CMTMRos and visualized by confocal fluorescence microscopy. c Populations of T cells with increased mitochondrial membrane potentials, quantified by flow cytometry. d, e ECAR (d) and OCR (e), measured using a Seahorse XFp analyzer. f–h Basal OCR (f), SRC (g) and OCR/ECAR h values, obtained based on ECAR and OCR values (*P < 0.05, **P < 0.01). i β-Hydroxybutyrate secretion levels (***P < 0.001)