Fig. 5.

Hormone metabolism and its effect on plant physiology. (A) Abscisic acid and gibberellin content in transplastomic lines. (B) Abscisic acid and gibberellin (GA₁ and GA₄) content in RNAi lines. Columns and bars represent the means and SEM of five biological replicates and three technical replicates. (C) Seed germination in DcLCYB1 transplastomic and NtLCYB RNAi lines (three independent plates, n=20). (D) Biomass of 10-day-old DcLCYB1 transplastomic and NtLCYB RNAi lines (three independent plates, n=6). (E) Root length of 10-day-old DcLCYB1 transplastomic and NtLCYB RNAi lines (three independent plates, n=6). (F) Hormone and inhibitor treatments in 10-day-old DcLCYB1 transplastomic lines. (G) Hormone and inhibitor treatments in 10-day-old NtLCYB RNAi lines. Tobacco seedlings grown in agar MS medium (10-day-old) were transferred to liquid MS medium (with agitation) and treated for 7 d with hormones (GA₃, GA₄, ABA, 1 µM; GA₃/ABA, 1 µM/0.66 µM; GA₄/ABA, 1 µM/0.66 µM) and inhibitors (paclobutrazol/PBZ, 1 µM). Biomass was calculated as percentage for each treatment normalized to wild type as 100% in each respective treatment (n=6 for each transgenic line and wild type). Unpaired two-tailed Student’s t-test was performed to compare transgenic lines with the wild type (*P<0.05, **P<0.001, ***P<0.0001). ABA, abscisic acid, GA, gibberellins, GA₃, gibberellin A₃; GA₄, gibberellin A₄; PBZ, paclobutrazol; WT, wild type.