Change of current value with changing voltage bias (100 mV) direction after a α-HL nanopore inserted into the lipid membrane with the entrance towards the cis (a) and the trans (b) compartment, respectively. Corresponding signature events at each α-HL orientation when DNA-AA@CB[6] was added to the cis side (c, d). Inserts depict the step-by-step molecular process of DNA hybrid trapping, dissociation, and translocation, as well as CB[6] oscillation in the vestibule. Data was acquired in 3 M KCl, 10 mM Tris buffer, pH 8.0, with the transmembrane potential held at +200 mV.