Figure 1.

Plan of the experimental protocol for a chronic chemotherapy‐induced cardiomyopathy with beta‐blockers. Ten‐week‐old C57Bl6 male mice were evaluated 2 days before the onset of chemotherapy, with measurements of body weight (BW) and cardiac parameters by transthoracic echocardiography (TTE). This evaluation was repeated on Days 21 and 38. Doxorubicin (Dox, n = 11) or saline (n = 9) was IP injected every 2 days (from Days 0 to 14), and 1 week later, mice received IP of either trastuzumab (Trast) or saline again for 2 weeks. In addition, 14 mice were treated with metoprolol (100 mg/kg) in the drinking water from Day 10 prior to the onset of the protocol with Dox + Trast (n = 8) or saline (n = 6) to the end. BW, body weight; TTE, transthoracic echocardiography.