Fig. 2. N3 dependent reaction monitoring the active site selectivity of inhibitors. (A) Reaction of N3 with the Mpro active site Cys-145.1 (B) N3, (C) ebselen and (D) TPCK modifies Mpro in a covalent manner. (E) Ebselen covalently modifies multiple Mpro cysteine residues in the presence of N3. (F) TPCK does not covalently modify Mpro in the presence of N3, suggesting that it selectively reacts with active site Cys-145. Black spectra: wild-type Mpro (33796 Da).
