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. 2021 Mar 25;220(6):e202007182. doi: 10.1083/jcb.202007182

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© 2021 Robertson et al.

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Figure 2. — ERM-deficient T cells have a cell-intrinsic egress defect. (a and b) Representative flow plots and total numbers of major thymocyte populations. (c–f) CD4 single-positive (c and d) and CD8 single-positive (e and f) populations were analyzed to determine the numbers of semi-mature (CD24^Hi S1PR1^Low) and mature (CD24^Low S1PR1^Hi) subpopulations. (e and f) Statistical significance for b, d, and f was determined by comparing the WT and DKO means using multiple t tests (two-sided) and the Holm-Sidak correction for multiple comparisons. Each dot represents an individual mouse; n = 4. Data distribution was assumed to be normal, but this was not formally tested. (g and h) Competitive in vivo migration. CD45 congenic WT and DKO naive CD4⁺ T cells labeled with CMFDA were coinjected 1:1 into the tail veins of WT recipient mice and harvested from the indicated tissues 1 or 24 h later. Each dot represents a recipient mouse; data are pooled from two independent experiments. Means ± SD are indicated with horizontal bars in d, f, g, and h. Dotted lines in g and h indicate a 1:1 ratio. DN, double negative; DP, double positive; SP, single positive; Ing. LN, inguinal lymph node; Mes. LN, mesenteric lymph node. *, P < 0.05; **, P < 0.01.