Figure 3.

DKO T cells have a selective transmigration defect toward S1P. (a) Transmigration to the indicated doses of S1P. Naive CD4⁺ T cells cultured for 1 h or overnight in media with CS-FBS, with or without pretreatment with 100 nM Ex26. Each data point represents the mean of technical replicates from one independent experiment (n = 4 experiments). (b–d) Percent transmigration of WT and DKO naive CD4⁺ T cells (previously cultured overnight in media with 10% CS-FBS) to the indicated doses of S1P (b), CCL19 (c), or CXCL12 (d). Each data point represents one technical replicate from one experiment, representative of three to five independent experiments. Means for each concentration of chemoattractant were compared with t tests (two-sided) using the Holm-Sidak correction for multiple comparisons. Data distribution was assumed to be normal, but this was not formally tested. **, P < 0.01.