FIGURE 13.

Fibroblast cell proliferation under the effect of Wnt signaling activation or inhibition. Cells were seeded in triplicate at a density of 10,000 cells/well in opaque 96-well plates for 24 h. The CellTiter 96^® Aqueous One solution cell proliferation assay was used to measure the viability of healthy and fibroblasts from asthmatic patients after 24 h of treatment with selective Wnt agonist and FH535, a small-molecule inhibitor of β-catenin/TCF/LEF. The 24-h values were normalized to their corresponding starting value (0-h incubation).