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. 2021 Mar 29;16(3):e0247595. doi: 10.1371/journal.pone.0247595

Fig 2. Establishment of Dox-inducible GATA1s or GATA1fl Ts21-ES cells.

Fig 2

(A) Scheme of the Dox-inducible GATA1s. (B) Parental clones and generated GATA1s or GATA1fl Dox-inducible subclones. The Dox-inducible GATA1s construct was knocked into AAVS1 locus with the CRISPR-Cas9 system, and the Dox-inducible GATA1fl construct was transduced by the PiggyBac system. (C) Western blot analysis of GATA1s and GATA1fl expression in untreated ESCs and ESCs treated with 1 μg/ml Dox for 24 h. K562 was used as the positive control.