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. 2021 Mar 17;17(3):e1009447. doi: 10.1371/journal.ppat.1009447

Fig 4. Viruses harboring mutated SZF1-binding sites spontaneously express lytic genes and demonstrate defects in B cell transformation.

Fig 4

(A) Peripheral blood mononuclear cells (PBMC) from three healthy donors were infected with wild-type or SZF1-binding site mutant p2089 viruses in the presence of FK506. Cells were counted using Trypan Blue at indicated time points and absolute live cells were plotted. Results were averaged between three donors. (B-D) PBMC from 3 donors infected as in (A) were harvested after 48 hours for RT-qPCR analysis of EBV lytic genes BZLF1, BGFL4, and BDLF2, relative to wild-type p2089-infected PBMC. Data represent averages from three independent experiments; error bars, SEM; *, p ≤ 0.05.