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. 2021 Mar 17;17(3):e1009447. doi: 10.1371/journal.ppat.1009447

Fig 8. SZF1 is enriched at oriP on the lytic genome but is not associated with actively replicating viral DNA.

Fig 8

A. Read coverage at EBV oriP. Plots show the read distributions at oriP under lytic and refractory conditions. Coverage of the reads from lytic and refractory genomes were determined with Bedtools software (v2.30.0) and plotted in R. Lytic reads were normalized to refractory EBV genome copy number, Rightward and leftward strands are indicated by blue and red, respectively. Genome position numbers corresponding to the reference genome NC_007605 are indicated. B-D. EBV-positive cells were untreated or exposed to NaB for 36 hours (or 24 hours in C) and subjected to (B) ChIP with anti-SZF1 antibody or control antibody followed by qPCR amplification of oriP and BZLF1p and analysis of data by normalizing to 2% input and IgG control, (C) isolation of intracellular DNA and qPCR amplification of BALF5 gene, or (D) isolation of proteins on nascent DNA (iPOND) followed by western blotting with indicated antibodies. Data in B and C represent averages of two independent experiments; error bars, SEM; ***, p < 0.001; iPOND was performed twice.