Table 2. Extracellular metabolite profiling of CCA cells.
| Metabolites | Chemical shift (ppm) | Control shRNA | CD44shRNA#1 | p-value | CD44shRNA#2 | p-value | |||
|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | ||||
| Acetate | 1.91(s); 1.92(s) | 80621411 | 29581977 | 77793510 | 11296723 | 0.89 | 81684694 | 1908510 | 0.96 |
| 5-Hydroxytryptamine | 3.12(t); 3.31(t); 7.1(s); 7.3(s); 7.42(d) | 9453840 | 309635 | 9565075 | 230053 | 0.65 | 9643188 | 389248 | 0.55 |
| Alanine | 1.48(d); 3.79(q) | 24838420 | 3043193 | 27763521 | 1430727 | 0.23 | 29358909 | 31434 | 0.12 |
| Alpha-aminoisobutyrate | 1.5(s) | 7551400 | 2223 | 8064715 | 38533 | 0.00 | 7611648 | 190329 | 0.64 |
| Choline | 3.21(s); 3.52(m); 4.07(m) | 19054547 | 711747 | 17442822 | 831861 | 0.06 | 15927649 | 539928 | 0.00 |
| Carnitine | 2.44(dd); 3.23(s); 3.43(m) | 7928277 | 6230001 | 8862367 | 5926882 | 0.86 | 14657051 | 1378963 | 0.20 |
| Cysteate | 3.29(dd); 3.55(dd);4.1(dd) | 14760560 | 1111762 | 10911037 | 1569558 | 0.03 | 16015323 | 2084060 | 0.42 |
| Cysteine | 3.03(dd);3.1(dd);3.97(t) | 5168590 | 114695 | 5496504 | 59810 | 0.02 | 5173434 | 80343 | 0.96 |
| Formate | 8.46(s) | 992562 | 200567 | 1131645 | 50819 | 0.35 | 1436960 | 27825 | 0.06 |
| Glutamate | 2.1(m); 2.36(d);3.77(t) | 10139242 | 1235737 | 9646621 | 1021934 | 0.62 | 9076097 | 391355 | 0.27 |
| Glutamine | 2.14(m); 2.46(m); 3.77(t) | 5055280 | 941146 | 5841647 | 686711 | 0.31 | 5238564 | 406770 | 0.78 |
| Homoserine | 2.03(m); 2.16(m); 3.79(m) | 28531052 | 378523 | 26039497 | 656844 | 0.01 | 28854845 | 2250261 | 0.83 |
| Isoleucine | 0.94(t); 1.01(d); 1.26(m); 1.48(m); 1.98(m); 3.68(d) | 31577921 | 2818006 | 30878412 | 1732803 | 0.74 | 27638507 | 354893 | 0.13 |
| Kynurenine | 3.72(d); 4.16(t); 6.82(t); 6.89(d);7.43(t); 7.89(d) | 10828166 | 124376 | 11046765 | 75891 | 0.07 | 10822015 | 254377 | 0.97 |
| Lactate | 1.33(d); 4.11(q) | 483437293 | 45780472 | 571290808 | 2378439 | 0.08 | 582492477 | 39725542 | 0.05 |
| Methionine | 2.14(s);2.16(m);2.65(t);3.86(t) | 2918861 | 12539 | 3071895 | 63100 | 0.05 | 2944615 | 139671 | 0.78 |
| N-Acetylhistamine | 2.84(t);3.45(m);7.03(s);7.95(s) | 3893415 | 166750 | 3940131 | 212722 | 0.78 | 4123136 | 156388 | 0.16 |
| Lysine | 1.48(m); 1.73(q); 1.91(m); 3.03(t); 3.76(t) | 15716527 | 1132939 | 17103076 | 1293122 | 0.24 | 16508687 | 228739 | 0.35 |
| Pyruvate | 2.38(s) | 14941174 | 4389969 | 13333639 | 5040858 | 0.70 | 9174226 | 566034 | 0.15 |
| Alpha-hydroxybutyrate | 0.9(t); 1.7 (m); 4.0(dd) | 11290706 | 2058758 | 10534176 | 2090266 | 0.68 | 9589419 | 995065 | 0.29 |
| p-Hydroxypheny-lacetate | 3.45(s);6.87(d);7.17(d) | 12081082 | 148156 | 12500655 | 112314 | 0.02 | 12403107 | 562972 | 0.43 |
| Threonine | 1.33(d);3.59(d);4.26(dq) | 5472008 | 585886 | 4988499 | 1017401 | 0.52 | 3970536 | 349497 | 0.03 |
| Tryptamine | 3.18(t); 3.35(t); 7.21(t); 7.29(t); 7.34(s); 7.56(d); 7.71(d) | 55098590 | 19499839 | 52738921 | 11129957 | 0.87 | 83057465 | 8047978 | 0.12 |
| Glucose | 3.69(m);3.82(m);8.78(s) | 5459404 | 2440785 | 3754747 | 276885 | 0.35 | 1695742 | 487485 | 0.11 |
| Valine | 0.99(d); 1.04(d); 2.28(dd); 3.62(d) | 38237289 | 338812 | 39865495 | 239507 | 0.00 | 38790628 | 1651787 | 0.62 |
(s) singlet; (d); doublet; (t) triplet; (q) quartet; (dd) doublet of doublet; (m) multiplet.
Data are expressed as mean ± S.D. from two-time independent experiments. p < 0.05 from t- test analysis.