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MC-38 colon cancer cell (A) and B16 melanoma cells (B) were exposed to different concentration of 1,25(OH)2D3 for 72 h. Cells proliferation was evaluated by SRB assay at the end of the experiment as the optical density (OD) of 1,25(OH)2D3-treated cells/ the optical density (OD) of 1,25(OH)2D3-untreated cells *100; bars represent the mean of cells proliferation ± SD; Unpaired t test ** p<0.005; ***p<0.0001.
