Table 1:
Key properties of High Five™, previously reported TnCLV-negative Tn, and Tn-NVN cell lines.
| Cell line | References | Growth in SFM | Productivity in SFM | Single cell suspension culture | Peak cell density [106 cells / mL] | Clonal | Species of origin confirmed | Tn-CLV free | Free of other adventitious agents | Genome / transcrtiptome available |
Free of immunogenic Coreα−3 fucosylated N-glycans? |
|---|---|---|---|---|---|---|---|---|---|---|---|
| BTI-Tn-5B1–4 (High Five™) | (R. R. Granados, 1991; Robert R. Granados, Guoxun, Derksen, & McKenna, 1994; Wickham et al., 1992) | YES | YES | NO1 | ~ 5 | YES | YES | NO | ? | YES (Fu et al., 2018)/ YES (Yu et al., 2016) | NO |
| MSU-TnT4 (TnT4) | (Thiem, 2011; Zhang, Manzan, Peplinski, & Thiem, 2008; Zhang & Thiem, 2010) | ? | ? | ? | < 1 | NO | NO2 | YES | ? | NO / NO | ? |
| QAU-BTI-Tn9–4s | (Meng, Li, Li, & Li, 2008; Zheng, Zhou, & Li, 2014) | YES | YES | YES | ~ 4.5 | NO | NO3 | YES | ? | NO / NO | ? |
| QB-CL-B | (Shan, Zhang, Jiang, Ma, & Li, 2011) | ? | ? | YES | 1.6 | YES | NO | ?4 | ? | NO / NO | ? |
| BTI-Tnao38 | (Y. Hashimoto et al., 2010; Yoshi Hashimoto et al., 2012) | YES | YES | YES5 | 2.5 | YES | YES | YES | ? | NO / NO | ? |
| Tnms42 | (Chen et al., 2014; Chen et al., 2013) | ? | ? | ? | ? | YES | YES6 | YES | ? | NO / YES (Chen et al., 2013) | ? |
| Tn NVN | This study | YES | YES | YES | 6.5 | YES | YES | YES | YES | YES / YES This study | YES |
Some labs have reportedly adapted High Five™ to single cell suspension cultures (D. Esposito, personal communication) (Rhiel et al., 1997; Mark A. Saarinen, Troutner, Gladden, Mitchell-Logean, & Murhammer, 1999), but without adaptation or polyanionic supplements, High Five cells typically form large aggregates when cultured in suspension.
DNA fingerprinting was used to determine TnT4 was genetically distinct from Sf21.
DNA fingerprinting was used to determine QAU-BTI-Tn9–4s is genetically similar to T. ni eggs and High Five™ cells, but distinct from Sf9.
QB-CL-B has not been assayed directly for TnCLV genetic material, but is likely TnCLV-negative because it is a clonal derivative of the TnCLV-negative QAU-BTI-Tn9–4s cell line.
“The majority of cells in the shaker culture appeared to be single suspended cells …” (Y. Hashimoto et al., 2010).
Cell line species of origin confirmed through transcriptomic sequences.