Figure 7.

Activation of cruzipain is a key process during metacyclogenesis. Quantification of relative number of epimastigotes, intermediate forms and metacyclic forms obtained at 72 h from parasites (Y WT strain) exposed to in vitro metacyclogenesis in absence or in presence of autophagy inhibitors (Baf or Wort), broad-spectrum protease inhibitors (Prot Inh) (A) or K777 drug (B), which were added during the complete differentiation cycle. Data are shown as the mean of three independent experiments. A total of 50 parasites per group for each experiment were quantified