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Cholesterol derivatives cooperate with nutrient signaling to regulate phosphorylation modification of BmRpd3. (A-C’) Protein levels of p-EIF4EBP1 after 20E (A), cholesterol (B) or 27-OH (C) treatment for 6 h. Quantification of p-EIF4EBP1, respectively in A (A’), B (B’), and C (C’). (D-F) mRNA level of BmMTORC1 (D), phosphorylation levels of immunoprecipitated BmRpd3-HA (E), and immunofluorescent staining of BmRpd3-HA (F) after BmMTORC1 RNAi treatment for 48 h in BmN cells
