Figure 2.

The AIEC strain LF82 catabolizes propionate

(a) Bacterial growth curves of the LF82 strain in M9 supplemented with 30 mM propionate or 10 mM glucose. (b) The AIEC strain LF82, the AIEC LF82ΔprpC isogenic mutant and the corresponding trans-complemented mutant LF82ΔprpC:prpC were grown in minimal medium supplemented with 30 mM propionate. After 24 h of incubation, the propionate concentration was measured. The experiment was performed in duplicate with n = 3 at each time. The results are the mean ± SEM. Statistical comparisons were carried out by one-way ANOVA with the Bonferroni post hoc test (*p < 0.05, **p < 0.01) after normality testing using the Kolmogorov-Smirnov test.