Figure 4.

AIEC LF82 decreases the propionate concentration in vivo.

CEABAC10 mice (n = 8 for each group) were pretreated with fosfomycin (2 g/L) for 4 days and orally infected with the LF82 strain or the isogenic mutant LF82ΔprpC. (a) Propionate concentrations in fecal samples of mice collected after infection with LF82 or LF82ΔprpC. For each mouse, fecal samples collected before infection with the LF82 strain were pooled, and the concentration of propionate was determined as 100%. (b) Secreted KC chemokine and (c) IL-6 cytokine amounts from colonic tissue of infected mice 2 days post infection. The results are presented as the median. Statistical comparisons were carried out by normality testing using Kolmogorov-Smirnov tests and a subsequent two-tailed Student’s t-test (*p < .05).