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. 2021 Mar 26;13(1):1902718. doi: 10.1080/19490976.2021.1902718

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um. (d) The relative mRNA level of mucin1, mucin2, ZO-1 and claudin-1 was detected in colon samples on day 3 after colitis induction. *p < .05, **p < .01, ***p < .001. All data were presented as the means ± SD (n = 6 mice per group)