Figure 1.

Substrate-binding pockets and Nsp10 binding interface of Nsp16 observed in the crystal structure of the Nsp16/Nsp10 complex (PDB: 6wks). (A) Surface representation of Nsp16 showing the SAM-binding pocket (cyan), RNA-binding pocket (yellow), and Nsp10-binding interface (green). (B) Overlay of Nsp16 structures from structures of the Nsp16/Nsp10 complex with RNA (PDB: 6wks, shown in gray) and without RNA (PDB: 6w4h, shown in cyan), showing structural heterogeneity in the RNA binding site. Gate loop 1 and gate loop 2 of the RNA-binding pocket, and SAM-binding loop 1 (SAMBL1) and SAM-binding loop 2 (SAMBL2) lining the SAM-binding pocket are highlighted. To see this figure in color, go online.