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. Author manuscript; available in PMC: 2021 Mar 29.
Published in final edited form as: Br J Haematol. 2019 Aug 6;187(5):666–675. doi: 10.1111/bjh.16129

Fig 5.

Fig 5.

Haem clearance is positively associated with Hmox1 induction and haem trafficking. (A) Nrf2-dependent, organ specific induction of haem importer Hrg1 (n = 4). (B) Haem importer Hrg1 expression is more highly induced by haem in liver, kidney and spleen of sickle cell mice (n = 3–10). (C) Change in haem content is correlated with haemin-induced Hrg1 expression in wild type mice (n = 3–4). For DeltaCt, lowest value = highest expression and highest value = lowest expression. Target gene transcripts were normalized to Gusb for all mRNA expression level. Gusb expression was similar in all mice strains used and in all of these organs in animals injected with either vehicle or haemin. For relative fold change, samples were further normalized to vehicle control gene transcripts. R2 was calculated with Pearson correlation. All values are mean ±SEM. *P < 0·05, **P < 0·01, ***P < 0.001. BM, bone marrow; H, heart; K, kidney; Li, liver; Lu, lung; S, spleen.