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. 2021 Mar 20;2021:6628957. doi: 10.1155/2021/6628957

Figure 4.

Figure 4

EA enhanced arginase activity and ARG 2 level in DOX-treated mice with reduced iNOS level. (a, b) The arginase activity in serum and cardiac tissues of Veh, DOX, and DOX+EA groups. (c–f) mRNA levels of ARG 2, iNOS, nNOS, and eNOS in cardiac tissues of Veh, DOX, and DOX+EA groups. (g) Representative images of ARG 2 and iNOS protein expression in cardiac tissues of each group. (h, i) Quantification of ARG 2 and iNOS protein expression in each group. β-Tubulin was used as the loading control. iNOS: inducible nitric oxide synthase; ARG 2: arginase 2; eNOS: endothelial nitric oxide synthase; nNOS: neuronal nitric oxide synthase. Values are presented as mean ± SEM, ∗P < 0.05 and ∗∗P < 0.01. n = 6 mice/group.