Table 1.
Lipid markers for male and female S. mansoni, determined via assessment of MS images using AP-SMALDI MSI (PC = phosphatidylcholine; TG = triacylglycerol; DG = diacylglycerol; SM = sphingomyelin). Exemplary signal distribution for these ions can be seen in ESM Fig. S13
| Sex of the adult worms | Measured m/z | Theoretical m/z | Error [ppm] | Annotated molecule | Sum formula of the ion |
|---|---|---|---|---|---|
| Female | 768.587717 | 768.587763 | − 0.06 | PC(O-34:1) | [C42H84NO7P + Na]+ |
| 746.605737 | 746.605818 | − 0.11 | PC(O-34:1) | [C42H84NO7P + H]+ | |
| 883.772498 | 883.772511 | − 0.02 | TG(52:1) | [C55H104O6 + Na]+ | |
| 579.534598 | 579.534686 | − 0.15 | DG(34:0) | [C37H72O5 + H – H2O]+ | |
| Male | 785.652905 | 785.653102 | − 0.25 | SM(40:2) | [C45H89N2O6P + H]+ |
| 783.637179 | 783.637452 | − 0.35 | SM(40:3) | [C45H87N2O6P + H]+ | |
| 703.574887 | 703.574852 | 0.05 | SM(34:1) | [C39H79N2O6P + H]+ | |
| 731.606070 | 731.606200 | − 0.11 | SM(36:1) | [C41H83N2O6P + H]+ | |
| 807.634818 | 807.635047 | − 0.28 | SM(40:2) | [C45H89N2O6P + Na]+ | |
| 805.619087 | 805.619397 | − 0.39 | SM(40:3) | [C45H87N2O6P + Na]+ |