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. 2021 Jan 21;124(7):1301–1311. doi: 10.1038/s41416-020-01250-4

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© The Author(s) 2021

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Fig. 5 — a Cell lysates from 293 T cells transfected with Flag-EEF1A2 were immunoprecipitated using anti-Flag agarose and visualised by Coomassie blue staining. Interacting proteins were identified by liquid chromatography mass spectrometry (LC-MS)/MS. b A549 cells were transfected with Flag-EEF1A2, and the proteins were immunoprecipitated with Flag monoclonal antibody. The HSP90AB1 and HSP90AA1 were detected by immunoblotting. c Confocal images showing expression of Flag-tagged EEF1A2 (green) and Myc-tagged HSP90AB1 (red) in A549 cells. Red and green images were acquired sequentially. Scale bar = 50 µm. d Domain organisation and co-immunoprecipitation analysis of three Flag-EEF1A2 truncation fragments (left), and three Myc-HSP90AB1 truncation fragments (right).