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. 2021 Mar 29;11:7070. doi: 10.1038/s41598-021-86486-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of culture medium composition on viability, response to pro-inflammatory stimuli and migration ability of macrophages in a 3D environment. (A) Representative maximum intensity projections of macrophages after culture in RPMI control (“RPMI ctrl”), skin differentiation 1 (“Skin diff. 1”) or skin differentiation 2 (“Skin diff. 2”) media, upon live/dead staining. Scale bars: 100 µm. (B) Quantification of macrophage viability. N = 3, n = 2. Error bars represent standard deviation. Statistical significance indicated with ***p < 0.001. (C) Representative 3D reconstructed images of live/dead staining after vertical invasion assay, color coded for invasion depth. (D) Quantification of macrophage maximal migration distance. N = 3, triplicate measurements. Error bars represent standard deviation. (E) Quantification of pro-inflammatory cytokines IL-6 and IL-8 secretion in supernatants. N = 3, duplicate measurements. Error bars represent standard deviation.