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. 2021 Mar 29;12:1952. doi: 10.1038/s41467-021-21694-3

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© The Author(s) 2021, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Stomatal aperture measurement of A. thaliana WT, almt9-2 and complementation lines. Epidermal strips were pre-incubated in stomatal measurement buffer for 1 h under dark, followed by a 1.5 h dark-to-light transition, as indicated above graphs by black (dark) or white (light) bars, ±2 mM GABA; n = 189 (control) and n = 195 (GABA) for WT-like 2 (segregated from almt9-2)²¹, n = 197 (control) and n = 153 (GABA) for almt9-2, n = 213 (control) and n = 178 (GABA) for almt9-2 complement with 35S::ALMT9 #1 (almt9-2/ALMT9 #1), n = 219 (control) and n = 127 (GABA) for almt9-2/ALMT9 #2, n = 195 (control) and n = 115 (GABA) for almt9-2 complemented with 35S::ALMT9 with double mutation F243C/Y245C (ALMT9^F243C/Y245C) targeting the putative GABA interaction residues^18,36,39 (almt9-2/F243C/Y245C #1), n = 221 (control) and n = 109 (GABA) for almt9-2/F243C/Y245C #2 with control treatment. b–g Leaf feeding assay of almt9-2 and complementation lines. Stomatal conductance of detached leaves from 5–6-week-old Arabidopsis almt9-2, almt9-2/ALMT9 #2 and almt9-2/F243C/Y245C #1 plants was recorded using a LI-COR LI-6400XT in response to dark (shaded region) and 200 µmol m⁻² s⁻¹ light (white region), fed with artificial xylem sap solutions ± 4 mM GABA (b, d, f). The iWUE of almt9-2 (c), almt9-2/ALMT9 #2 (e) and almt9-2/F243C/Y245C #1 (g) detached leaves was calculated based on the ratio of photosynthetic rate (Supplementary Fig. 17b, e, h) versus stomatal conductance (b, d, f); n = 14 (control) and n = 13 (GABA) for almt9-2 (b, c); n = 15 (control and GABA) for almt9-2/ALMT9 #2 (d, e); n = 13 (control) and n = 12 (GABA) for almt9-2/F243C/Y245C #1 (f, g). All data are plotted with box and whiskers plots: whiskers plot represents minimum and maximum values, and box plot represents second quartile, median and third quartile (a, c, e, g), or data are represented as mean ± s.e.m (b, d, f); statistical difference was determined by two-sided Student’s t test; *P < 0.05, **P < 0.01, ****P < 0.0001 (a–g).