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. 2021 Mar 25;14:1085–1110. doi: 10.2147/JIR.S294630

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© 2021 Wang et al.

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Effect of a restrain stress on the barrier function in the colon. (A) Time-dependent changes in FITC-dextran 4000 (FD-4) flux through all subgroups as paracellular pathway for 120 mins. (B) Horseradish peroxidase (HRP) flux (=transcellular pathway) was measured ex vivo. (C) Western blot for TJ proteins. Distal colon was subjected to immunoblotting for ZO-1, occludin, claudin-5 and GAPDH. Representative results of one experiment are shown. Similar results were obtained in three independent experiments: y-WKY group, y-WKY-S group, y-SHR group, y-SHR-S group. (D–F) The intensity of the bands was quantified by scanning densitometry, standardized with respect to ZO-1, occludin, claudin-5 and GAPDH protein and expressed as mean ± SEM. Unpaired t-test, *p < 0.05, ***p < 0.001 (Blue y-WKY-S vs y-WKY, Green y-SHR vs y-WKY); ^#p < 0.05, ^##p < 0.01 (y-SHR-S vs y-SHR); ^Δp < 0.05, ^ΔΔp < 0.01 (Black y-SHR-S vs y-WKY-S, Green y-SHR vs y-WKY-S).