Table 3.

Primer sequences for detection of bla_CTX-M, bla_SHV, bla_TEM, bla_IMP, bla_VIM genes and gyrB.

Target gene	Primers	Primers sequences (5′–3′)	Tm in 0 °C	References
blaCTX-M	CTX-M forward primer	ATGAGYACCAGTAARGTKATGGC	58.7	45
	CTX-M reverse primer	ATCACKCGGRTCGCCIGGRAT	59.3	45
	CTX-M Probe	FAM-CCCGACAGCTGGGAGACGAAACGT-BBQ	70.2	45
blaSHV	SHV forward primer	TCCCATGATGAGCACCTTTAAA	56.8	46
	SHV reverse primer	TCCTGCTGGCGATAGTGGAT	58.6	46
	SHV Probe	FAM-TGCCGGTGACGAACAGCTGGAG-BBQ	68.3	46
blaTEM	TEM forward primer	GCATCTTACGGATGGCATGA	56.6	46
	TEM reverse primer	GTCCTCCGATCGTTGTCAGAA	57.7	46
	TEM Probe	FAM-CAGTGCTGCCATAACCATGAGTGA-BHQ1	62.2	46
blaIMP	IMP forward primer	GGGCGGAATAGAGTGGCTTA	57.6	47
	IMP reverse primer	GGCTTGAACCTTACCGTCTTTTT	59.3	47
	IMP Probe	FAM-CGATCTATCCCCACGTATGCATCTGAATTAACA-BHQ1	67.4	47
blaVIM	VIM forward primer	TGCGCTTCGGTCCAGTAGA	59.0	47
	VIM reverse primer	TGACGGGACGTATACAACCAGAT	58.5	47
	VIM Probe	FAM-CTTCTATCCTGGTGCTGCGCATTCG-BHQ1	67.6	47
gyrB	gyrB forward primer	CCT GAC CAT CCG TCG CCA CAA		48
	gyrB reverse primer	CGC AGC AGG ATG CCG ACG CC		48
	gyrB probe	6-FAM-CCG TGG TGG TAG ACC TGT TCC CAG ACC-BHQ 6-FAM-CCG TGG TGG TAG ACC TGT TCC CAG ACC-BBQ		This study