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. 2021 Mar 29;12(4):334. doi: 10.1038/s41419-021-03574-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A ATF4 expression profile across all tumor samples and corresponding normal tissues in the TCGA database. B Combined analysis of ATF4 expression in pancreatic cancer tissues and corresponding normal tissues from TCGA and GTEx databases. C qRT-PCR analysis of ATF4 expression levels in 60 PDAC tissue samples and paired normal adjacent tissue samples collected in our hospital. ^***p < 0.001. D qRT-PCR assay was performed to detect the mRNA expression of ATF4 in the pancreatic normal ductal epithelial cell line HPDE6-C7 and cancer lines including AsPC-1, BxPC-3, Capan-2, CFPAC-1, MIA PaCa-2, PANC-1, and SW1990. n = 3. E Western blot assay showing the protein expression level of ATF4 in a pancreatic normal ductal epithelial cell line and cancer cell lines. F Representative IHC staining images showed negative (−), weakly positive (+), moderate positive (++), and strongly positive (+++) expression of ATF4 in pancreatic cancer tissue samples. G Kaplan–Meier survival analysis was performed to compare ATF4-high and ATF4-low PDAC patients’ progression-free survival times from our hospital. N = 52, log-rank test. H Kaplan–Meier survival analysis was performed to compare ATF4-high and ATF4-low PDAC patients’ overall survival times from our hospital. N = 52, log-rank test.