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. 2021 Mar 29;11:7113. doi: 10.1038/s41598-021-86464-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of microbiome beta-diversity across conditions based on ASV features using non-metric dimensional scaling (NMDS). Ellipses representing centroid position for each group, showing the distribution of samples and groups along the primary axis (NMDS1), closely aligned with a gradient in microbial alpha-diversity (A); Dirichlet multinomial mixture (DMM) groups defined through differences in patterns of abundance were well separated along the primary X axis and gradient in alpha-diversity (B).