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Capture of low p24 concentrations. (A) Recombinant p24 at the lowest concentrations of the standard curve was completely recovered after IP, and (B) p24 concentration matches between direct Simoa and IP-Simoa after sample volume normalization. (C) The IP-Simoa assay’s lower limit of detection (LOD) is ≤0.005 pg/mL defined as the coefficient variation (CV) <20% for each p24 standard. This would equate to 1 fg of p24 concentrated from a given volume and released into 200 μL for assay.
