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. 2021 Mar 16;8:625347. doi: 10.3389/fvets.2021.625347

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Copyright © 2021 Quiroga, Alarcón, Manosalva, Teuber, Taubert, Hermosilla, Hidalgo, Carretta and Burgos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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D-Lactate treatment increases the intracellular levels of both lactate stereoisomers as well as the L-lactate dehydrogenase subunit A (L-LDHA) expression. bFLS were treated with 5 mM D-lactate for 1 h. (A) L-lactate and (B) D-lactate were quantified at the intracellular level by high-performance liquid chromatography (HPLC). Each bar represents the mean ± SEM. Each point represents an independent experiment, n = 5. (C) The relative mRNA expression of L-LDHA was assessed by RT-qPCR. Bovine tumore necrosis factor-α (TNF-α) was used as the positive control. Each bar represents the mean ± SEM, n = 5. *p < 0.05; **p < 0.01.