Table 1.

Target genes, forward and reverse primer (5′-3′) sequences, amplicon size (pb), regression coefficient (R²) value, slope, and amplification efficiency (%).

Gene	Forward primer, 5′-3′	Reverse primer, 5′-3′	Size (bp)	R2	Slope	Efficiency (%)
rps9	GCTGACGCTGGATGAGAAAGACCC	ATCCAGCACCCCGATACGGACG	85	0.995	−3.612	89.164
il-6	ACTGGCAGAAAATAAGCTGAATCTTC	TGATCAAGCAAATCGCCTGAT	89	0.998	−3.523	92.239
il-8	ATGACTTCCAAGCTGGCTGTTG	TTGATAAATTTGGGGTGGAAAG	149	0.998	−2.920	120.040
hif-1α	GGAGTTGGACCTCTGCGATT	GAGGGGAGAAAAGGCACGTC	102	0.995	−3.267	102.331
glut-1	GCGGACCCTACGTCTTCATC	GGCCTTTTGTCTCGGGAACT	87	0.999	−3.304	100.749
pdk-1	CTCATCGGAAACACGTCGGA	TCACACAGACGCCTAGCATT	91	0.996	−3.484	93.643
l-ldha	AGGCCTGAGAAGTCGGAGTG	GGAACCTGTCCTACCTGCC	118	0.983	−3.512	92.633

rps9, 40S ribosomal protein S9; il-6, interleukin 6; il-8: interleukin 8; hif-1α, hypoxia inducible factor-1 subunit alpha; glut-1, solute carrier family 2 (facilitated glucose transporter), member 1; pdk-1, pyruvate dehydrogenase kinase 1; l-ldha, L-lactate dehydrogenase subunit A; bp, base pairs.