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. 2021 Mar 29;12:1940. doi: 10.1038/s41467-021-22173-5

Fig. 3. MTHFD2 is induced by IFN-γ and involved in IFN-γ-mediated PD-L1 regulation.

Fig. 3

a, b Indicated cancer cells transfected with control or MTHFD2 siRNAs for 24 h and then treated with 0 or 20 ng/ml IFN-γ for another 24 h, PD-L1 mRNA levels were analyzed by real-time PCR (a) and immunoblotting analyses were performed using the indicated antibodies (b). c, d Indicated cancer cells pretreated with LY294002 (c) and Rapamycin (d) with indicated concentrations and then cultured with 20 ng/ml IFN-γ, immunoblotting analyses were performed using the indicated antibodies. e Indicated cancer cells treated with 20 ng/ml IFN-γ for indicated times, immunoblotting analyses were performed using the indicated antibodies. In (a), the values are presented as mean ± s.e.m (n = 3 independent experiments); p values (Student’s t test, two-sided) with control or the indicated groups are presented (also see Supplementary Fig. 3).