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. 2021 Mar 16;9:645658. doi: 10.3389/fcell.2021.645658

FIGURE 3.

FIGURE 3

Galectin-14 promotes the expression of N-cadherin and matrix metalloproteinase (MMP)-9, Akt phosphorylation, and activity of MMP-9 in trophoblasts. (A,C) Primary extravillous trophoblasts (EVTs) were transfected with scramble siRNA or siRNA targeting galectin-14. (B,D) HTR-8/Svneo was transfected with empty vehicle or vector containing galectin-14 gene. (A,B) Protein expression was assessed by Western blotting with antibodies against VE-cadherin, N-cadherin, MMP-9, tissue inhibitor matrix metalloproteinase (TIMP)-1, MMP-2, TIMP-2, p-Akt, Akt, and galectin-14 and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (C,D) Gelatinase activity was determined by gelatin zymography. Results are presented as mean ± SEM of biological triplicates. Statistical analysis: Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. si-Ctrl, cells transfected with scramble siRNA; gal-14-siRNA, cells transfected with siRNA targeting galectin-14; WT, HTR-8/SVneo transfected with empty vehicle; OE, galectin-14-overexpressed HTR-8/SVneo.