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. 2021 Mar 16;9:645658. doi: 10.3389/fcell.2021.645658

FIGURE 7.

FIGURE 7

Galectin-14 promotes matrix metalloproteinase (MMP)-9 expression and activity through enhancing Akt phosphorylation. In galectin-14-overexpressed HTR-8/SVneo, 10 μM SC79 or 10 μM LY294002 was added as the agonist or inhibitor of Akt phosphorylation. (A) Protein expression was assessed by Western blotting with antibodies against VE-cadherin, N-cadherin, MMP-9, tissue inhibitor matrix metalloproteinase (TIMP)-1, MMP-2, TIMP-2, p-Akt, Akt, and galectin-14 and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (B) Gelatinase activity was determined by gelatin zymography. Results are presented as mean ± SEM of biological triplicates. Statistical analysis: Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. WT, HTR-8/SVneo transfected with empty vehicle; OE, galectin-14-overexpressed HTR-8/SVneo. Dimethylsulfoxide (DMSO), cells added with DMSO of the same volume; SC79, cells added with 10 μM SC79; LY294002, cells added with 10 μM LY294002.