TABLE 4.

The extracellular proteases secreted by strain 1A02591 identified by secretome analysis.

Gene ID	Family	Protease	PSMs	Abundancea	Biological functions
orf00069	M4	Thermostable neutral proteinase	50	15.72%	Degrades extracellular proteins and peptides for bacterial nutrition; thermolysin-like protease
orf04095	S8	Serine protease	46	14.47%	Thermostable peptidase
orf02484	M3	M3 family	46	14.47%	Degradation of oligopeptides
orf02323	M3	Oligoendopeptidase F	37	11.64%	Degradation of oligopeptides
orf03925	S11	D-alanyl-D-alanine carboxypeptidase	31	9.75%	Synthesis of bacterial cell walls, cleaving the D-Ala-D-Ala crosslinks in the cell wall peptidoglycans
orf00077	M20	Dipeptidase PepV	30	9.43%	Hydrolyses the late products of protein degradation so as to complete the conversion of proteins to free amino acids
orf03187	M32	Carboxypeptidase M32	24	7.55%	Stable up to high temperatures
orf00831	C40	Peptidase P60	23	7.23%	Responsible for the degradation of bacterial cell wall components
orf02670	M23	M23 family peptidase	12	3.77%	Lyses cell walls of other bacteria, either as a defensive or feeding mechanism
orf03370	S8	Serine protease	10	3.14%	Thermostable peptidases
orf02654	S41	Peptidase S41	7	2.20%	C-terminal processing peptidase
orf03626	C40	Peptidase P60	2	0.63%	Responsible for the degradation of bacterial cell wall components

^aAbundance was calculated based on the proportion of the PSMs of a protease in the sum of PSMs of all proteases in the secretome.