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. 2021 Mar 16;11:637710. doi: 10.3389/fcimb.2021.637710

Figure 1.

Figure 1

Susceptibility of human astrocytes to ZIKV and its pathologic effects on infected cells. (A) U251 cells were infected with ZIKV SZ01 at MOI of 1 and cytopathic effects (CPE) were observed at 24, 48, and 72 h p.i. (Magnification x40). (B) ZIKV E protein was expressed and detected by immunofluorescence assay with a specific antibody for E protein using confocal microscopy. (C) Replication of ZIKV genome in the infected cells. ZIKV E gene copies were quantified at various time points p.i. by real-time RT-PCR with specific primers for the viral E gene. Data were representative of three experiments and each experiment was performed in triplicate. Unpaired Student’s T-test was used to analyze the differences of data and error bars represent standard errors of the means. (D) Expressed viral E protein was detected in the infected cells by western blot with specific antibody (1:1000) for the E protein. (E) Viral titers in U251 cells infected with ZIKV at various MOI. Culture supernatants from the infected cells was collected at indicated time point p.i. and the virus titrated in BHK21 cells by a standard plaque assay (*p < 0.05; ***p < 0.001).