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. 2021 Mar 16;11:637710. doi: 10.3389/fcimb.2021.637710

Figure 2.

Figure 2

Cell death and reduced viability observed in human astrocytes infected with ZIKV. (A) U251 cells with or without ZIKV infection (MOI of 1) co-stained with Annexin V and PI at 48 and 72 h p.i. for flowcytometric analyses. (B) Quantitative analyses of cell death at various time points p.i. in U251 cells infected with ZIKV. ***p < 0.001. (C) Cell viability of U251 cells infected with ZIKV at various MOI was assessed with an MTT assay. Triplicate cultures were analyzed at the indicated time points (*p < 0.05; **p < 0.01; ***p < 0.001).