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(A) Comparison of different sample preparation protocols across all 47 bacterial isolates, (including 3 biological replicates and 4 technical replicates for each strain and protocol) and (B) age of the bacterial colony (47 bacterial isolates, 4 technical replicates for each strain and day) for spectra acquired on a microflex Biotyper MALDI-TOF MS system. ‘****’p-value < 0.0001.
