Table 2.
Primers used in viral load determination
| Gene | Gene Accession Number | Primers (5′-3′) |
|---|---|---|
|
CVSA (Outer Primer) |
KR105374.1 | Forward: 5’-GGCACAGTCGTCACCGCTTA-3’ |
| Reverse: 5’-TGAGGGGCACATGCAGCAAT-3’ | ||
|
RAV (Outer Primer) |
NM_008828.3 | Forward: 5’-GCTCTGGGCTGGTGTCGTTC-3’ |
| Reverse: 5’-TACGGGGACTTCCCGCTCAG-3’ | ||
|
CVSN (Nested Primer) |
KR105374.1 |
Forward: 5’-AGAAGAATGTTCGAGCCAGGGGCAAG-3’ |
|
Reverse: 5’-AGGAGACTTCCCACTCAAGCCTAGTG-3’ |
||
|
RAVN (Nested Primer) |
KR080523.1 |
Forward: 5’-AGAATGTTCGAGCCAGGGCAGGAC-3’ Reverse: 5’-ACTTCCCGCTCAGACCCAACGAG-3’ |
*CVSA, CVSN: Used for amplification of CVS-11 viral strain
*RAV, RAVN: Used for amplification of SRV viral strain