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. 2021 Mar 16;11:619346. doi: 10.3389/fonc.2021.619346

Figure 2.

Figure 2

(A) The cell migration ability of lung cancer cells (XWLC-05, YTMLC-90, and NCI-H157) after miR-34a up-regulation was detected by wound healing assay. Representative images of wound healing area in each group under a light microscope (magnification, x40; scale bar, 200 µm); (B) Histograms indicated the migration rate of each group at 24 and 48 h after transfection. Cell migration was detected using the wound healing assays. Uniform scratches were made in XWLC-05, YTMLC-90, and NCI-H157 cells and the serial photographs were obtained at different time points posttransfection. The scratch width for the cells transfected with pGCMV/EGFP-hsa-miR-34a plasmid was decreased dramatically compared to the pGCMV/EGFP-hsa-miR-NC plasmid transfection negative control group and the blank control group (n = 3, ****P < 0.0001 respectively versus the corresponding control). (C) The cell invasion ability of lung cancer cells (XWLC-05, YTMLC-90, and NCI-H157) after miR-34a up-regulation was detected by the trans-well invasion assay. Representative images of the invasive cells in each group under a light microscope (magnification, ×100; scale bar, 100 µm). The invasive cells were stained with 0.1% crystal violet and appeared in purple; (D) Histograms indicated the invasion rate of each group at 48 h after transfection. The average number of the invasive cells was calculated from five random views. The average number of the invasive cells in the pGCMV/EGFP-hsa-miR-34a plasmid group was decreased dramatically compared to the pGCMV/EGFP-hsa-miR-NC plasmid transfection negative group and the blank control group (n = 3, *p < 0.05, ***P < 0.001, ****P < 0.0001 respectively versus the corresponding control). ns, statistical significance exist.