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. 2021 Mar 16;12:642711. doi: 10.3389/fimmu.2021.642711

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Copyright © 2021 Hendriks, Mnich, Clemente, Cruz, Tavarini, Bagnoli and Soldaini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of T cell subsets in cell suspensions obtained from human skin explants. (A) Schematic overview of the protocol used to obtain single cell suspensions from human skin explants. (B) Gating strategy to analyze T cell subsets, γδ-, CD4⁺, and CD8⁺ T cells, in single cell suspensions. (C) Representative histograms (in red) showing the surface expression for skin resident memory T cells markers, CD45RO (memory), CLA (skin homing), and CD69 (tissue retention) on live CD4⁺ cells. Blue histograms represent unstained cells.